Kasama and Uesugi previously published a one-shot in October 2019 under the same title on Shonen Jump+ as part of a collaboration between Shueisha and Marvel Comics to publish a biweekly series of seven manga short stories.
Kasama and Uesugi launched the Deadpool: Samurai manga on Shueisha’s Shonen Jump+ website in December 2020 as a short serialization.
Viz Media released the manga as part of its collaboration with Marvel Comics to publish manga and comics based on Marvel characters.
Shueisha shipped the second and final compiled book volume in Japan in July 2021.