The film earned 277 million yen (about US$2.5 million) and sold 199,000 tickets on Saturday and Sunday.
The film had sold 315,000 tickets to earn 367 million yen (about US$3.42 million) in its opening weekend.
The film is a sequel of the series’ 20th film, Detective Conan: The Darkest Nightmare, and centers on Tōru “Zero” Amuro.
Meitantei Conan Zero no Shikkōnin (Detective Conan: Zero the Enforcer), the 22nd film in the Detective Conan series, remained at #1 at the box office in Japan in its fifth consecutive weekend.